Dextran (150?kDa) conjugated to FITC was purchased from TdB. the listeriolysin O (LLO)-mediated resealing technique, to deliver mid-sized, membrane-impermeable biopharmaceuticals into cells. We found that LLO-type resealing required no exogenous cytosol to repair the injured cell membrane and allowed the specific entry of mid-sized molecules into cells. We use this method to introduce either a … Continue reading Dextran (150?kDa) conjugated to FITC was purchased from TdB